cd206 rabbit polyclonal antibody  (Proteintech)

Journal: bioRxiv

Article Title: Siglec-engaging immunosuppressive sialoglycans are upregulated in prostate cancer and are targetable to suppress bone metastasis

doi: 10.1101/2025.11.12.687981

Figure Lengend Snippet: Dot plots of Siglec gene expression levels at single cell resolution in prostate tumours using previously published single cell RNA-sequencing data from ( A ) primary prostate tumours and ( B ) prostate cancer bone metastatic tissues . ( C ) Dual immunofluorescence analysis of Siglec receptors and immune cell markers in independent prostate cancer bone metastasis tissue samples. This analysis was also carried out for primary prostate tissue (Supplementary Figure 4). Siglec-7, -9, -10 and -15 are co-expressed with CD14 (a myeloid marker) and CD206 (an M2 macrophage marker) in bone metastatic tumours. Furthermore, we reveal Siglec-3 and -15 are expressed with cathepsin K (an osteoclast marker). Scale bar is 20□µm.

Article Snippet: Tissues were then blocked with 1X CFB for 1 hr at room temperature, following overnight incubation in the dark at 4 °C in 1:400 CD22 Monoclonal Antibody (Proteintech, 66103-1-Ig), 1:200 Anti-CD33 Antibody [WM53] (Abcam, ab252263), 1:200 Siglec-7/CD328 Polyclonal Antibody (Proteintech, 13939-1-AP), 1:200 Siglec-9 Polyclonal Antibody (Proteintech, 13377-1-AP), 1:100 Siglec-10 Polyclonal Antibody (Invitrogen, PA5-55501), 1:100 Siglec-15 Polyclonal Antibody (Abcam, ab198684), 1:50 Mouse Siglec-E Antibody (R&D Systems, AF5806), 1:200 CD14 Rabbit Polyclonal Antibody (Proteintech, 17000-1-AP), 1:200 CD14 Mouse Monoclonal Antibody (Proteintech, 60253-1-Ig), 1:500 AMACR/p504S Rabbit Polyclonal Antibody (Proteintech, 15918-1-AP), 1:200 AMACR Mouse Monoclonal Antibody [UMAB68] (UltraMAB, UM870012), 1:200 CD206 Rabbit Polyclonal Antibody (Proteintech, 18704-1-AP), 1:200 CD206 Mouse Monoclonal Antibody (Proteintech, 60143-1-Ig), 1:200 Cathepsin K Rabbit Polyclonal Antibody (Proteintech, 11239-1-AP), 1:200 Cathepsin K Mouse Monoclonal Antibody [E-7] (Santa Cruz, sc-48353), 1:200 CD20 Rabbit Polyclonal Antibody (Proteintech, 24828-1-AP) or 1:100 Pan Cytokeratin Monoclonal Antibody (AE1/AE3), Alexa FluorTM 488-conjugated (Invitrogen 53-9003-80).

Techniques: Gene Expression, RNA Sequencing, Immunofluorescence, Marker

Journal: Frontiers in Immunology

Article Title: Ganoderma lucidum spore powder alleviates rheumatoid arthritis-associated pain hypersensitivity through inhibiting accumulation, N1 polarization, and ROS production of neutrophils in mice

doi: 10.3389/fimmu.2025.1569295

Figure Lengend Snippet: GLSP treatment reversed the N1-polarized state of neutrophils in the affected paws of CIA mice. (A–C) GLSP treatment reduced both the proportion and absolute count of N1-polarized (CD95 + ) neutrophils in the paws of mice with CIA. (D–F) GLSP treatment upregulated both the proportion and number of N2-state (CD206 + ) neutrophils within the paws of CIA mice. (G, H) In vehicle-treated CIA mice, N1 polarization of joint neutrophils exhibits a negative correlation with mechanical and thermal pain thresholds. (I, J) In GLSP-treated CIA mice, N1 polarization of joint neutrophils demonstrates no correlation with mechanical or thermal pain thresholds. (K, L) In vehicle-treated CIA mice, N2 polarization of joint neutrophils shows no significant correlation with mechanical or thermal pain thresholds. (M, N) N2 polarization of joint neutrophils in GLSP-treated CIA mice correlates positively with both mechanical and thermal pain thresholds. Data are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001, one-way ANOVA assay followed by Tukey’s post hoc test (B, C, E, F) , Pearson correlation analysis (G–N) . NS, no significance.

Article Snippet: Rabbit polyclonal CD95 antibody (Proteintech, 1:1000 dilution), rabbit polyclonal CD206 antibody (Proteintech, 1:1000 dilution), and mouse polyclonal β-actin antibody (Proteintech, 1:1000 dilution) were used as primary antibodies.

Techniques:

Journal: Frontiers in Immunology

Article Title: Ganoderma lucidum spore powder alleviates rheumatoid arthritis-associated pain hypersensitivity through inhibiting accumulation, N1 polarization, and ROS production of neutrophils in mice

doi: 10.3389/fimmu.2025.1569295

Figure Lengend Snippet: GLSP attenuates TNF-α-induced nociceptive effects in neutrophils by reversing their N1 polarization. (A) Schematic representation of the experimental protocol designed to investigate the nociceptive effects of neutrophil N1 and N2 polarization states. (B, C) TNF-α challenge increased the proportion of CD95 + neutrophils in dHL-60 cells, an effect reversed by Co-treatment with GLSP extract, as shown by flow cytometry. (D, E) Co-treatment with GLSP extract reversed the TNF-α-challenged reduction in the proportion of CD206 + neutrophils in dHL-60 cells. (F, G) Intraplantar injection of TNF-α-challenged neutrophils induced mechanical allodynia and thermal hyperalgesia. Co-treatment with GLSP extract attenuated this effect. Data are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001, one-way ANOVA assay followed by Tukey’s post hoc test (C, E) , two-way ANOVA assay followed by Tukey’s post hoc test (F, G) . NS, no significance.

Article Snippet: Rabbit polyclonal CD95 antibody (Proteintech, 1:1000 dilution), rabbit polyclonal CD206 antibody (Proteintech, 1:1000 dilution), and mouse polyclonal β-actin antibody (Proteintech, 1:1000 dilution) were used as primary antibodies.

Techniques: Flow Cytometry, Injection